Fig 2: Comparison of hub targets by molecular docking and verification. (A) Bar graph of hub protein mean score values of the top ten docking scores. (B) 3D docking conformation of AZOX with AKT. Green dashed lines represent H-bonds and the numbers denote the distance of the H-bonds. (C) 3D docking conformation of AZOX with PI3K. Green dashed lines represent H-bonds and the numbers denote the distance of the H-bonds. (D) CETSA and Western blot analysis showed that AZOX may directly bind to AKT and PI3K.

Fig 3: Expressions of PI3K, p-PI3K, AKT, and p-AKT in chondrocytes. Chondrocytes were pretreated with 50, 100, or 200 µg/ml of GBT recipe or 100 nmol/ml DEX for 1 hour and then stimulated with 100 ng/ml LPS for 12 h. The data were derived from three independent experiments and expressed as mean ± standard deviation (*p < 0.05 compared with the control group; #p < 0.05 compared with the LPS-treated group).

Fig 4: LY294002 (a PI3K inhibitor) inhibited the phosphorylation of PI3K, AKT, and VEGFR2 in follicle microvascular endothelial-like cells of chicken (FMECs) induced by Angelica sinensis (AS) extract. ß-actin was used as an internal reference protein. All experiments were performed in triplicate, and the data are the mean ± S.E.M (*P < 0.05, **P < 0.01, ***P < 0.001).

Fig 5: 4-HD induced apoptosis and cycle arrest of HCC cells by regulating the PI3K/AKT/mTOR signaling pathway. (A,B) Cell apoptosis proportion treated with 4-HD+LY294002 was detected by flow cytometry; (C,D) The effects of LY294002 on the expressions of Bax, Bcl-2, cytochrome c, caspase-9 and caspase-3 and PARP in HepG2 cells treated with 4-HD were evaluated by Western blotting; (E,F) Cell cycle distribution proportion treated with 4-HD+LY294002 was determined by flow cytometry; (G,H) Effects of LY294002 on the expressions of cyclin B1 and CDK1/CDC2 proteins in HepG2 cells treated with 4-HD were assessed by Western blotting. Relative expressions of the proteins were normalized to GAPDH. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control (0 µM); # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. 40 µM 4-HD.